Abstract

The biosynthetic methods have been recognized as an alternative to chemical and physical synthesis, as this biosynthetic method is economical, ecofriendly, and cost-effective. The present work exhibited an efficient and low-cost biological approach to synthesize the metal nanoparticles and provided helpful insight into the development of new antimicrobial agents with the synergistic enhancement of the antimicrobial mechanism against pathogenic microorganisms.

A green synthesis of Zinc Oxide nanoparticles was carried out using Vitis vinifera skin peel extract. Synthesized nanoparticles were characterized using UV-Vis absorption spectroscopy, DLS, TEM, FT-IR and XRD studies respectively. UV-Vis absorption spectroscopy of prepared Zinc Oxide colloidal solution showed absorption maxima at 230nm. TEM analysis showed average particle size ranging from 0.5-10μm, while XRD confirms SAED pattern confirmed the crystalline nature of synthesized nanoparticles. FT-IR analysis revealed that primary and secondary amine groups in combination with the protein present in the peel extract are responsible for reduction reaction and also the stabilization of Zno nanoparticles. DLS shows the particle size of nanoparticles is 64.4nm and has a negative zeta potential of -34.1mV. Hence from this study we concluded that the nanoparticles synthesized from Vitis vinifera peel extract has shown very effective on in-vitro antimicrobial activity (Gram positive and negative Bacteria, Fungi) using disc diffusion method.

Keywords: Vitis vinifera; Zinc oxide nanoparticles; Antimicrobial activity